Part:BBa_K3016031:Design

Vibrio natriegens' native tatC CDS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 516
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The translocation complex requires also TatA and TatB subunits to function normally.

Source

Vibrio natriegens ATCC 14048 / DSM 759 genome. (GenBank NZ_CP016345.1)

References

Alanen, H. I., Walker, K. L., Suberbie, M. L. V., Matos, C. F., Bönisch, S., Freedman, R. B., ... & Robinson, C. (2015). Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1853(3), 756-763.

Browning, D. F., Richards, K. L., Peswani, A. R., Roobol, J., Busby, S. J., & Robinson, C. (2017). Escherichia coli “TatExpress” strains super‐secrete human growth hormone into the bacterial periplasm by the Tat pathway. Biotechnology and bioengineering, 114(12), 2828-2836.

Jack, R. L., Sargent, F., Berks, B. C., Sawers, G., & Palmer, T. (2001). Constitutive expression of Escherichia coli tat genes indicates an important role for the twin-arginine translocase during aerobic and anaerobic growth. Journal of bacteriology, 183(5), 1801-1804.